Control Plate

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biology DNA recombinant question?

If accidentally given you plates that contained only LB and X-gal, but no ampicillin,what would be result of TE control and uncut pGEM control plate? can anyone give the explanation

The key is to think about the role of the Ampicillin. It's an antibiotic, so it kills bacteria. The plasmid, pGEM, contains a gene that gives the bacteria resistance to Ampicillin. So, if you do the experiment normally, the only bacteria which will grow on the plate are those which contain the plasmid.

However, if your plates have no Ampicillin, then all the bacteria will grow on the plate - even those without pGEM. The result is that the plate will be completely overgrown with bacteria.

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