Air Acetylene

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How to calculate Concentration?

A 5 mL sample of blood was treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting solution was extracted with methyl isobutyl ketone containing the organic Pb-complexing agent APCD. This extract was aspirated into an air/acetylene flame yielding an absorbance of 0.24 at 283.3 nm. A 5 aliquot of sample was spiked with 0.02 mL of a 1 mg/L Pb and this gave an absorbance of 0.414. Calculate the concentration of Pb in the sample, assuming that Beer's Law applies

Use standard addition:

Cx=(Ax*Cs*Vs)/(Vx(As-Ax))

first we must calculate Cs:

m=1mg/L*0.00002L = 0.00002mg

Cs = m/V = 0.00002mg/0.005L = 0.004 mg/L

now in the equation:

Cx = (0.24*0.004*(0.005+0.0002))/(0.005(0.414-0.24))

Cx = 0.005539 mg/L

or at least it should be

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